An interesting concept is that the feral Egyptian Mau in Egypt today are more purebred that the finest purebred Egyptian Maus in the United States and Europe.
Moataz Ahmed Abd Elghaffar is a young Egyptian scientist who has a keen interest in answering some fundamental questions about the Egyptian Mau. In a proposed study his objectives are to:
- Prove that native Maus in modern Egypt are similar to the ancient Maus;
- Prove that Maus in Egypt are purer than overseas Maus and to
- Establish the differences and similarities between native Maus and overseas Maus.
He intends to analyze:
- The overseas Mau in Texas, USA
- Native Mau in Cairo, Egypt and the
- Mummified Cats of Ancient Egypt.
In outline the procedure for testing the hair of a mummified Eyptian Mau is as follows:
- Cut 10- 15 hair roots about 0.5 cm into a 1.5ml eppendorf tube.
- Use 50 ul of the following lysis buffer: 10mM Tris pH 8.3, 50mM KCL, 0.5% Tween
- Also add 10ul of 20ug/ml solution of Proteinase K in 10mM Tris-HCL (pH 7.5)
- Vortex for 30 seconds.
- Ultracentrifuge at 13000 rpmfor 1 second.
- Incubate overnight in a 56 - 60oC waterbath.
- Incubate for 10 minutes at 94oC ( to denature the proteinase K I presume)
- Cool down to room temperature.
- Ultracentrifuge at 13000 rpm for 1 second.
- Ready DNA for your PCR
I look forward to hearing about the results. It is said that there is no trace of the original Persian cat in the modern Persian cat. It is likely that there is no trace of the original British cats in the modern British Shorthair and American Shorthair. Selective breeding has seen to that. I wonder how original any of the modern cat breeds are.